Separation of lipids by Silica Gel G column chromatography

Separation of lipids by Silica Gel G column chromatography.

A column chromatographic procedure utilizing Silica Gel C is described for separating lipid components of serum and lipoproteins into individual fractions containing hydrocarbons (I), cholesterol esters (II), triglycerides (111), cholesterol (IV) , free fatty acids (V) , and phospholipids (VI). Silica Gel G required no pretreatment except adjustment of moisture content to 10%. The method afford...

Separation and Quantification of Hydrocarbons of LPG Using Novel MWCNT-Silica Gel Nanocomposite as Packed Column Adsorbent of Gas Chromatography

In this study, a new silica gel based adsorbent was fabricated and its ability in separation and quantification of alkanes mixture was investigated. Silica gel (SiO2) is a polar absorbent which is mainly used to separate polar compounds. Also, the carbon materials such as activated carbon and recently carbon nanotube (CNTs), have been widely used for separation of nonpolar materials. Carbon nan...

Chromatography of Lipids on Commercial Silica Gel Loaded Filter Paper.

Separation of aflatoxins by column chromatography.

Several column chromatographic methods are available for the separation of aflatoxins (l-6). Most of these procedures are elaborate and involve several steps of purification. A rapid and simple method of obtaining pure aflatoxins was reported by Steyn (5); however, with this method, we were not able to obtain pure crystalline aflatoxins B, and G1 for our experimental studies on animals. When pu...

Separation of folic acid compounds by gel chromatography on Sephadex G-15 and G-25.

The separation of folate compounds has been achieved by gel chromatography and the effect of buffer concentration on the elution pattern of folate derivatives was investigated by Sephadex G-15 and G-25 column chromatography. Highly conjugated polyglutamates appear in the effluent considerably sooner when eluted with water than would be expected from their molecular weights. Monoglutamates, exce...